12 labours of hercules iv level 3.83/6/2023 ![]() It has been reported that pregnant women with GDM are susceptible to pre-eclampsia 3, and face an increased risk of birth through cesarean section 6. Over the past 20 years, the incidence of gestational diabetes mellitus (GDM) has increased to as high as 17.5–18.9% in the Chinese population 1, 2, and this is of urgent concern, as GDM causes both short- and long-term health consequences in fetuses and their mothers 3- 5. These findings suggest that miRNA-125b and miRNA-144 are consistently dysregulated in circulating exosomes and the placenta from GDM pregnancies, and are of excellent diagnostic value for GDM. The area under curve for the established diagnostic model was 0.898, which was higher than blood glucose levels at 0 h. miRNA-144 levels in the circulating exosomes negatively correlated with body mass index both before pregnancy ( P = 0.018) and before delivery ( P = 0.039), and positively correlated with blood glucose at 1 h, estimated using the oral glucose tolerance test ( P = 0.044). The patterns of these two miRNAs remained the same in circulating exosomes from GDM pregnancies (all P < 0.001). Of these, miRNA-125b was downregulated ( P < 0.001), whereas miRNA-144 was upregulated ( P < 0.001). We identified 157 dysregulated miRNAs in the placental tissue obtained from GDM pregnancies. Univariate logistic regression analysis was used to establish diagnostic models based on miRNAs expression, and the diagnostic value was estimated using the receiver operator characteristic curve. Western blot and transmission electron microscopy were used to validate exosomes. Quantitative polymerase chain reaction was used to validate the identified targets. Next-generation sequencing was used to identify miRNAs in the placenta that were differentially expressed between GDM and normal glucose tolerance pregnancies. In a cross-sectional study, we identified miRNAs expressed both in the placenta and circulating exosomes of pregnant women with GDM, and estimated their diagnostic value. ![]() Differentially expressed microribonucleic acids (miRNAs) in the placenta and circulating exosomes are of diagnostic value for gestational diabetes mellitus (GDM).
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |